血管緊張素轉(zhuǎn)化酶(ACE)活性蛋白
Active Angiotensin I Converting Enzyme (ACE)
CD143; ACE1; DCP1; ACEI; ACE-I; Kininase II; Angiotensin-Converting Enzyme; Peptidyl-Dipeptidase A; Dipeptidyl Carboxypeptidase 1; Angiotensin-converting enzyme, soluble form
- 編號UAPA004Hu61
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 5% Trehalose.
- 性狀凍干粉
- 純度> 90%
- 等電點(diǎn)6.1
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價(jià)格 暫無報(bào)價(jià) 暫無報(bào)價(jià) 暫無報(bào)價(jià) 暫無報(bào)價(jià) 暫無報(bào)價(jià)
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
Angiotensin I Converting Enzyme (ACE)?is a zinc-dependent metalloprotease that plays a crucial role in the?renin-angiotensin system (RAS), regulating blood pressure and fluid balance. ACE converts the inactive?angiotensin I?into the potent vasoconstrictor?angiotensin II, which increases blood pressure by constricting blood vessels and stimulating aldosterone secretion. Additionally, ACE degrades?bradykinin, a vasodilator, further promoting hypertension.Besides,Renin (REN) has been identified as an interactor of ACE, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human ACE and recombinant human REN . Briefly, biotin-linked ACE were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μl were then transferred to REN-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μl stop solution to the wells and read at 450nm immediately. The binding activity of ACE and REN was shown in Figure 1, the EC50?for this effect is1.035μg/mL.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過一個(gè)月，-80°C不超過12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA004Hu01 | 血管緊張素轉(zhuǎn)化酶(ACE)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UEPA004Hu61 | 血管緊張素轉(zhuǎn)化酶(ACE)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA004Hu02 | 血管緊張素轉(zhuǎn)化酶(ACE)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA004Hu02 | 血管緊張素轉(zhuǎn)化酶(ACE)活性蛋白 | Cell?culture;?Activity?Assays. |
| UAPA004Hu61 | 血管緊張素轉(zhuǎn)化酶(ACE)活性蛋白 | Cell?culture;?Activity?Assays. |
| UPAA004Hu01 | 血管緊張素轉(zhuǎn)化酶(ACE)多克隆抗體 | WB; IHC |
| UPAA004Hu02 | 血管緊張素轉(zhuǎn)化酶(ACE)多克隆抗體 | WB |
| ULAA004Hu81 | 血管緊張素轉(zhuǎn)化酶(ACE)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| ULAA004Hu71 | 血管緊張素轉(zhuǎn)化酶(ACE)多克隆抗體(生物素標(biāo)記) | IHC |
| UMAA004Hu22 | 血管緊張素轉(zhuǎn)化酶(ACE)單克隆抗體 | WB; IHC; ICC; IP. |
| UMAA004Hu21 | 血管緊張素轉(zhuǎn)化酶(ACE)單克隆抗體 | WB |
| USEA004Hu | 血管緊張素轉(zhuǎn)化酶(ACE)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCA004Hu | 血管緊張素轉(zhuǎn)化酶(ACE)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA004Hu | 血管緊張素轉(zhuǎn)化酶(ACE)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Lipids in Health and disease | Association of elevated blood pressure and impaired vasorelaxation in experimental Sprague-Dawley rats fed with heated vegetable oil [PubMed: PMC2914008] |
| Malaysian Journal of Medical Sciences | Effect of Repeatedly Heated Palm Olein on Blood Pressure–Regulating Enzymes Activity and Lipid Peroxidation in Rats [Usm: MJMS19120] |
| Journal of Cancer Therapy | Plasma Levels of Angiotensin-Converting Enzymes 1 and 2 and AGTR2 (T1247G and A5235G) Gene Polymorphisms Are Associated to Breast Cancer Progression. [Scirp: Source] |
| The American journal of clinical nutrition | Vascular function and atherosclerosis progression after 1 y of flavonoid intake in statin-treated postmenopausal women with type 2 diabetes: a double-blind randomized controlled trial. [Pubmed: 23553151] |
| Journal of Vascular Surgery | Cathepsin G deficiency reduces periaortic calcium chloride injury-induced abdominal aortic aneurysms in mice [Pubmed:25037606] |
| Reprod Toxicol.? | ACE2 activation by xanthenone prevents leptin-induced increases in blood pressure and proteinuria during pregnancy in Sprague-Dawley rats [Pubmed:25205467] |
| Chemical Research in Toxicology | Sub-chronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwall Carbon Nanotubes by Intratracheal Instillation [Pubmed:25580880] |
| Chem Biol Interact | Arsenic causes aortic dysfunction and systemic hypertension in rats: Augmentation of angiotensin II signaling [PubMed: 26079204] |
| Biomed Res Int | Protective Effect of Enalapril against Methionine-Enriched Diet-Induced Hypertension: Role of Endoplasmic Reticulum and Oxidative Stress [PubMed: 26640794] |
| Clinics (Sao Paulo) | Mechanisms [PubMed: 26602523] |
| Chemical Research in Toxicology | Subchronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwalled Carbon Nanotubes by Intratracheal Instillation [PubMed: 25580880] |
| Intensive Care Medicine Experimental | Angiotensin converting enzymes in patients with acute respiratory distress syndrome [Content: 3] |
| Evid Based Complement Alternat Med. | Garlic Attenuates Plasma and Kidney ACE-1 and AngII Modulations in Early Streptozotocin-Induced Diabetic Rats: Renal Clearance and Blood Pressure Implications [Pubmed:27293465] |
| Вавиловский журнал генетики и селекции | Ренин-ангиотензин-альдостероновая система у крыс линии НИСАГ (ISIAH) со стресс-индуцированной артериальной гипертензией [30_Dubinina.pdf] |
| Animal Biology | The effect of heat stress on bull sperm quality and related HSPs expression [content:journals] |
| biomedicine & pharmacotherapy | Citrus leaf extract reduces blood pressure and vascular damage in repeatedly heated palm oil diet-Induced hypertensive rats. [pubmed:28068636] |
| Biomedicine & Pharmacotherapy | Parkia speciosa empty pod prevents hypertension and cardiac damage in rats given N(G)-nitro- L -arginine methyl ester [pubmed:28992471] |
| Journal of Functional Foods | Antihypertensive effects of?Ocimum gratissimumextract: Angiotensin-converting enzyme inhibitor in vitro and in vivo investigation [10.1016/j.jff.2017.05.033] |
| Molecules | Protective Effect of Polyphenol-Rich Extract from Bee Pollen in a High-Fat Diet [Pubmed:29614743] |
| Journal of?Surgical?Research | Impact of angiotensin-converting enzyme 2 levels on postoperative pneumonia after esophagectomy [Pubmed:29506841] |
| Intensive Care Medicine | Angiotensin-converting enzymes in acute respiratory distress syndrome [] |
| International Journal of Biological Sciences | Vascular Protection of TPE-CA on Hyperhomocysteinemia-induced Vascular Endothelial Dysfunction Through AA Metabolism Modulated CYPs Pathway [] |
| Medicine | Alteration and association between serum ACE2/angiotensin (1-7)/Mas axis and oxidative stress in chronic kidney disease: A pilot study [Pubmed: 32756181] |
| American Journal of Respiratory and Critical Care Medicine | Equilibrium Angiotensin Metabolite Profiling in Patients with Acute Respiratory Distress Syndrome Indicates Angiotensin Converting Enzyme Inhibition [Pubmed: 32628511] |
| Int Immunopharmacol | Crocin protects against cardiotoxicity induced by doxorubicin through TLR-2/NF-κB signal pathway in vivo and vitro [Pubmed: 32388215] |
| Food Bioscience | Djulis (Chenopodium formosanum) and its bioactive compounds affect vasodilation, angiotensin converting enzyme activity, and hypertension [] |
| Journal of Extracellular Vesicles | MiR155-5p in adventitial fibroblasts-derived extracellular vesicles inhibits vascular smooth muscle cell proliferation via suppressing angiotensin-converting enzyme?… [] |
| Biotechnology and Pharmacy | Chrysin pretreatment improves mitochondrial enzymes and angiotensin converting enzymes in L-NAME induced hypertensive rats [] |
| Dikkat Eksikli?i Hiperaktivite Bozuklu?u Tan?s? Olan Anjiyotensin I, II, III, Alamandin peptid, Anjiyotensin D?nü?türücü Enzim (ACE), NADPH Oksidaz Enzim Serum?… [] |
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