白介素17(IL17)活性蛋白
Active Interleukin 17 (IL17)
IL17A; CTLA8; IL-17A; Cytotoxic T-Lymphocyte-Associated Protein 8
- 編號(hào)UAPA063Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 97%
- 等電點(diǎn)9.0
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價(jià)格 ¥ 502 ¥ 1254 ¥ 2508 ¥ 7524 ¥ 18810
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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SDS-PAGE圖
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Figure. Western Blot; Sample: Recombinant IL17, Human.
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
IL17 (interleukin 17) is a member of IL17 cytokine family, which is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. It is reported that IL17 is a ligand for IL17RA, indicating its interaction with IL17RA. Thus, a binding ELISA assay was constructed to detect the association of recombinant human IL17 with recombinant human IL17RA. Briefly, IL17 were diluted serially in PBS with 0.1%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL17RA-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-IL17 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution , wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of IL17 with IL17RA was shown in Figure 1 and this effect was in a dose dependent manner.
用法
Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過一個(gè)月，-80°C不超過12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UEPA063Hu61 | 白介素17(IL17)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA063Hu01 | 白介素17(IL17)活性蛋白 | Cell?culture;?Activity?Assays. |
| UEPA063Hu62 | 白介素17(IL17)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA063Hu01 | 白介素17(IL17)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA063Hu06 | 白介素17(IL17)多克隆抗體 | WB; IHC; ICC; IP. |
| UPAA063Hu01 | 白介素17(IL17)多克隆抗體 | WB |
| URAA063Hu21 | 白介素17(IL17)重組抗體 | WB; IF; ICC; IHC; IP; FCM. |
| UMAA063Hu21 | 白介素17(IL17)單克隆抗體 | WB |
| UMAA063Hu22 | 白介素17(IL17)單克隆抗體 | IHC |
| UMAA063Hu23 | 白介素17(IL17)單克隆抗體 | IHC |
| UMAA063Hu24 | 白介素17(IL17)單克隆抗體 | IHC |
| UMAA063Hu25 | 白介素17(IL17)單克隆抗體 | IHC |
| UFAA063Hu12 | 抗白介素17(IL17)單克隆抗體(標(biāo)記) | FCM |
| UHEA063Hu | 白介素17(IL17)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UMEA063Hu | 白介素17(IL17)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USEA063Hu | 白介素17(IL17)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCA063Hu | 白介素17(IL17)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA063Hu | 白介素17(IL17)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA063Hu01 | 白介素17(IL17)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It(ELISA Kit) Yourself" |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
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| Biomedical Research | Associations among interleukin-17, interferon-gamma, and acute coronary syndrome [ISSN 0970-938X] |
| Cellular Physiology and Biochemistry | Activated Α7nachr Improves Postoperative Cognitive Dysfunction and Intestinal Injury Induced by Cardiopulmonary Bypass in Rats: Inhibition of the Proinflammatory?… [Pubmed:29672286] |
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| Biology?of?Reproduction | Recombinant adiponectin alleviates abortion in mice by regulating Th17/Treg imbalance via p38MAPK-STAT5 pathway [Pubmed: 30496353] |
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| Minocycline Alleviate A Rat Model Of Gulf War Illness Via Altering Gut Microbiome, Attenuating Neuroinflammation And Enhancing Hippocampal Neurogenesis [] | |
| 中國(guó)應(yīng)用生理學(xué)雜志 | 丹參對(duì)非酒精性脂肪肝Th17細(xì)胞及相關(guān)細(xì)胞因子的影響* [] |
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