不對稱二甲基精氨酸(ADMA)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Asymmetrical Dimethylarginine (ADMA)
N,N-Dimethylarginine; NG,NG-Dimethylarginine dihydrochloride; Asymmetric Dimethylarginine
- 編號UKSB301Ge11
- 物種Pan-species (General，通用) 相同的名稱，不同的物種。
- 試劑內(nèi)容 包被抗體、生物素標(biāo)記的競爭物、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 可適用原理競爭抑制酶聯(lián)免疫吸附試驗(yàn)法測抗原
- 可檢測范圍24.69-2,000ng/mL
- 可適靈敏度8.98ng/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價(jià)格 ¥ 4363 ¥ 7272 ¥ 13090 ¥ 25452 ¥ 43632
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對不對稱二甲基精氨酸(ADMA)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。
儲存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時(shí)間為1個(gè)月。
配套試劑
贈(zèng)品
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相關(guān)產(chǎn)品
| 編號 | 適用物種：Pan-species (General，通用) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UCPB301Ge11 | 不對稱二甲基精氨酸(ADMA)牛血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UCPB301Ge21 | 不對稱二甲基精氨酸(ADMA)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UPAB301Ge01 | 不對稱二甲基精氨酸(ADMA)多克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| ULAB301Ge71 | 不對稱二甲基精氨酸(ADMA)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| UCEB301Ge | 不對稱二甲基精氨酸(ADMA)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMB301Ge | 不對稱二甲基精氨酸(ADMA)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSB301Ge11 | 不對稱二甲基精氨酸(ADMA)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| PLoS One. | Interactions among Vascular-Tone Modulators Contribute to High Altitude Pulmonary Edema and Augmented Vasoreactivity in Highlanders [PubMed: PMC3439466] |
| Cytokine | Effect of CCL5 on dimethylarginine dimethylaminohydrolase-1 production in vascular smooth muscle cells from spontaneously hypertensive rats. [Pubmed: 23834755] |
| Neurol Res. | Effect of 18β-glycyrrhetinic acid on cerebral vasospasm caused by asymmetric dimethylarginine after experimental subarachnoid hemorrhage in rats. [Pubmed:25475507] |
| Int J Clin Exp Med. | Correlation research on ADMA plasma levels and left ventricular function of peritoneal dialysis patients [Pubmed:25550968] |
| Clin Respir J | Asymmetric dimethylarginine contributes to airway nitric oxide deficiency in patients with COPD [PubMed: 26076870] |
| Cytokine | Dimethylarginine dimethylaminohydrolase-1 mediates inhibitory effect of interleukin-10 on angiotensin II-induced hypertensive effects in vascular smooth muscle cells of spontaneously hypertensive rats [PubMed: 26375520] |
| Neurological Research | Effect of 18β-glycyrrhetinic acid on cerebral vasospasm caused by asymmetric dimethylarginine after experimental subarachnoid hemorrhage in rats [PubMed: 25475507] |
| Scientific Reports | Dimethylarginine Dimethylaminohydrolase 2 (DDAH 2) Gene Polymorphism, Asymmetric Dimethylarginine (ADMA) Concentrations, and Risk of Coronary Artery Disease: A Case-Control Study [pubmed:27677852] |
| clinica chimica acta | Increased serum concentrations of asymmetric dimethylarginine (ADMA) in patients with early-onset coronary artery disease. [pubmed:27884754] |
| The?Journal?of?Physiology | Asymmetric?dimethylarginine?(ADMA)?elevation?and?arginase?up-regulation?contribute?toendothelial?dysfunction?related?to?insulin?resistance?in?rats?and morbidly obese humans. [pubmed:26840628] |
| Angiogenesis | Dimethylarginine dimethylaminohydrolase-1 (DDAH1) is frequently upregulated in prostate cancer, and its overexpression conveys tumor growth and angiogenesis by metabolizing asymmetric dimethylarginine (ADMA). [pubmed:29150732] |
| Scientific Reports | A Novel Role of Irbesartan in Gastroprotection against Indomethacin-Induced Gastric Injury in Rats: Targeting DDAH/ADMA and EGFR/ERK Signaling [Pubmed:29523851] |
| Mechanisms of?Ageing?and Development | Multivessel analysis of progressive vascular aging in the rat: Asynchronous vulnerability among vascular territories [Pubmed:29605639] |
| Nutrition, Metabolism and Cardiovascular Diseases | Arginine bioavailability and endothelin-1 system in the regulation of vascular function of umbilical vein endothelial cells from intrauterine growth restricted newborns [Doi: 10.1016/j.numecd.2018.09.002] |
| Biochemical?and?Biophysical?Research?Communications | Probucol improves erectile function via Activation of Nrf2 and coordinates the HO-1/DDAH/PPAR-γ/eNOS pathways in streptozotocin-induced diabetic rats [Pubmed: 30454888] |
| Brazilian?Journal?of?Pharmaceutical?Science | Demographic, clinical and lifestyle predictors for severity of erectile dysfunction and biomarkers level in Malaysian patients [] |
| ACS?Combinatorial?Science | Novel cellularly active inhibitor regresses DDAH1 induced prostate tumor growth by restraining tumor angiogenesis through targeting DDAH1/ADMA/NOS pathway [Pubmed: 30673277] |
| International Journal of the Cardiovascular Academy | Nebivolol prevents the increase of asymmetric dimethylarginine and oxidants in hyperhomocysteinemic rats [] |
| Oxid Med Cell Longev | The Role of Reactive Oxygen Species and Nitric Oxide in the Inhibition of Trichophyton rubrum Growth by HaCaT Cells [Pubmed: 32104540] |
| Molecular Medicine Reports | Blueberry anthocyanin?enriched extract ameliorates transverse aortic constriction?induced myocardial dysfunction via the DDAH1/ADMA/NO signaling pathway in?… [Pubmed: 31746378] |
| International Journal of Nephrology | Effect of a Supervised Peridialytic Exercise Program on Serum Asymmetric Dimethylarginine in Maintenance Hemodialysis Patients [Pubmed: 33163233] |
| Journal of Clinical Medicine | The Usefulness of Serum Biomarkers in the Early Stages of Diabetic Retinopathy: Results of the EUROCONDOR Clinical Trial [Pubmed: 32344735] |
| Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub | Levels of endothelial substances in patients with newly identified hypertension compared with healthy controls [33463628] |
| WIENER KLINISCHE WOCHENSCHRIFT | Non-functioning adrenal incidentalomas may increase toxic metabolites [34255169] |
| pharmaceuticals | Effect of a Low Dose of Carvedilol on Cyclophosphamide-Induced Urinary Toxicity in Rats—A Comparison with Mesna [34959638] |
| Int J Biochem Cell Biol | HIF-1α and Nrf2 regulates hypoxia induced overexpression of DDAH1 through promoter activation in prostate cancer [Pubmed:35644470] |
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