干擾素β(IFNb)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Interferon Beta (IFNb)
IFNB1; IFN-B; IFB; IFF; IFNB; Interferon Beta 1 Fibroblast
- 編號(hào)UKSA222Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 試劑內(nèi)容 包被抗體、檢測(cè)抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測(cè)樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測(cè)抗原
- 可檢測(cè)范圍7.8-500pg/mL
- 可適靈敏度2.7pg/mL
- 應(yīng)用自己動(dòng)手制作ELISA試劑盒的主要材料
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價(jià)格 ¥ 3024 ¥ 5040 ¥ 9072 ¥ 17640 ¥ 30240
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對(duì)干擾素β(IFNb)具有高靈敏度和高特異性，經(jīng)檢測(cè)與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時(shí)間為1個(gè)月。
配套試劑
贈(zèng)品
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA222Hu02 | 干擾素β(IFNb)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA222Hu01 | 干擾素β(IFNb)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPA222Hu01 | 干擾素β(IFNb)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA222Hu01 | 干擾素β(IFNb)多克隆抗體 | IHC |
| UPAA222Hu02 | 干擾素β(IFNb)多克隆抗體 | WB; IHC |
| UMAA222Hu22 | 干擾素β(IFNb)單克隆抗體 | WB; IHC |
| UMAA222Hu21 | 干擾素β(IFNb)單克隆抗體 | IHC |
| UMAA222Hu23 | 干擾素β(IFNb)單克隆抗體 | IHC |
| UMAA222Hu24 | 干擾素β(IFNb)單克隆抗體 | IHC |
| UMAA222Hu25 | 干擾素β(IFNb)單克隆抗體 | IHC |
| UMAA222Hu26 | 干擾素β(IFNb)單克隆抗體 | WB; IHC; ICC; IP. |
| UMAA222Hu29 | 干擾素β(IFNb)單克隆抗體 | ICC/IF |
| UMAA222Hu27 | 干擾素β(IFNb)單克隆抗體 | WB; IHC; ICC; IP. |
| USEA222Hu | 干擾素β(IFNb)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UMEA222Hu | 干擾素β(IFNb)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UAEA222Hu | 抗干擾素β抗體(Anti-IFNb)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
| ULMA222Hu | 干擾素β(IFNb)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA222Hu01 | 干擾素β(IFNb)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | 自己動(dòng)手制作ELISA試劑盒的主要材料 |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| 20 | Melatonin protects liver against ischemia and reperfusion injury through inhibition of toll-like receptor signaling pathway [PubMed: 21355876] |
| Journal of General Virology | Hepatitis B virus polymerase inhibits RIG-I- and Toll-like receptor 3-mediated beta interferon induction in human hepatocytes through interference with interferon regulatory factor 3 activation and dampening of the interaction between TBK1/IKKε and DDX3. [Sgm: 9182080] |
| Biochemical and Biophysical Research Communications | Impaired TLR3/IFN-β signaling in monocyte-derived dendritic cells from patients with acute-on-chronic hepatitis B liver failure: Relevance to the severity of liver damage [ScienceDirect: S0006291X09019822] |
| PLoS One | Intact Type I Interferon Production and IRF7 Function in Sooty Mangabeys [PubMed: PMC3757038] |
| PLoS Neglected Tropical Diseases | Innate Immune Response to Rift Valley Fever Virus in Goats [PubMed: PMC3335883] |
| BioMed Research International | Low pH Environmental Stress Inhibits LPS and LTA-Stimulated Proinflammatory Cytokine Production in Rat Alveolar Macrophages [Pubmed: 24288685] |
| Vet Microbiol. | An attenuated EIAV strain and its molecular clone strain differentially induce the expression of Toll-like receptors and type-I interferons in equine monocyte-derived macrophages. [Pubmed: 23850441] |
| Food and Chemical Toxicology | Scoparone attenuates d-galactosamine/lipopolysaccharide-induced fulminant hepatic failure through inhibition of toll-like receptor 4 signaling in mice [Pubmed: 23535186] |
| Veterinary immunology and immunopathology | Experimental model of equine alveolar macrophage stimulation with TLR ligands [Pubmed: 23815824] |
| American journal of respiratory cell and molecular biology | Training Modifies Innate Immune Responses in Blood Monocytes and in Pulmonary Alveolar Macrophages [Pubmed: 24502337] |
| FEBS Lett. | Mannan binding lectin attenuates double-stranded RNA-mediated TLR3 activation and innate immunity [Pubmed:24530528] |
| Veterinary Research | Infection of equine monocyte-derived macrophages with an attenuated equine infectious anemia virus (EIAV) strain induces a strong resistance to the infection by a virulent EIAV strain [Pubmed:25106750] |
| PLOS Neglected Tropical Diseases | MicroRNA-30e* Suppresses Dengue Virus Replication by Promoting NF-κB–Dependent IFN Production [Pubmed:25122182] |
| Veterinary Research | The innate immune response of equine bronchial epithelial cells is altered by training [Pubmed:25595212] |
| molecular and celluar biology | SOCS3 Drives Proteasomal Degradation of TBK1 and Negatively Regulates Antiviral Innate Immunity [PubMed: 25939384] |
| Arch Virol | Analysis of cytokine production in a newly developed canine tracheal epithelial cell line infected with H3N3 canine influenza virus [PubMed: 25797196] |
| J | Complexes of DNA with the Antimicrobial Peptide LL37 Augment NK Cell Functions by Inducing Type I Interferon Production from Circulating Monocytes and Plasmacytoid Predendritic Cells [PubMed: 26258404] |
| Virus | PKR activation enhances replication of classical swine fever virus in PK-15 cells [PubMed: 25899421] |
| PLoS?One | siRNA Targeting the 2A pro Genomic Region Prevents Enterovirus 71 Replication In Vitro [Pubmed:26886455] |
| Scientific Reports | Cellular uptake of exogenous calcineurin B is dependent on TLR4/MD2/CD14 complexes, and CnB is an endogenous ligand of TLR4 [Pubmed:27090571] |
| Front Microbiol | Innate Immune Responses in ALV-J Infected Chicks and Chickens with Hemangioma In Vivo [Pubmed:27252695] |
| Canadian Journal of Physiology and Pharmacology | Chloroquine attenuates lipopolysaccharide-induced inflammatory responses through upregulation of USP25. [pubmed:28134560] |
| International Journal of Molecular Medicine | Effects of IRF1 and IFN-β interaction on the M1 polarization of macrophages and its antitumor function [pubmed:27176664] |
| International Journal of?Clinical?and Experimental Pathology | Host miR-146a promotes replication of human cytomegalovirus by suppressing type I IFN response in MRC-5 cells [files:ijcep0036479.pdf] |
| Poultry?Science | ALV-J strain SCAU-HN06 induces innate immune responses in chicken primary monocyte-derived macrophages [pubmed:27486255] |
| Journal?of?Virology | Characterization?of?Simian?Immunodeficiency?Virus?Variants?Anatomically?Compartmentalized?inPlasma?and?Milk?in?Chronically?Infected?African?Green?Monkeys. [pubmed:] |
| Biochemical and Biophysical Research Communications | MicroRNA-548j inhibits type I interferon production by targeting ZBTB11 in patients with chronic hepatitis B [pubmed:28342861] |
| Virology | The down-regulation of casein kinase 1 alpha as a host defense response against infectious bursal disease virus infection [pubmed:28988058] |
| EMBO?Journal | Zika virus elicits inflammation to evade antiviral response by cleaving cGAS via NS1‐caspase‐1 axis [Pubmed:30065070] |
| Toxicology and?applied?pharmacology | Bisphenol A induced male germ cell apoptosis via IFNβ-XAF1-XIAP pathway in adult mice [Pubmed:30017639] |
| Archives of Virology | MAVS induces a host cell defense to inhibit CSFV infection [Pubmed:29556776] |
| Journal?of?virology | Inhibition of DNA-sensing pathway by Marek's disease virus VP23 protein through suppression of interferon regulatory factor 7 activation [Pubmed: 30518647] |
| Veterinary Immunology and Immunopathology | Molecular cloning and functional characterization of porcine 2′, 5′-oligoadenylate synthetase 1b and its effect on infection with porcine reproductive and respiratory?… [Doi: 10.1016/j.vetimm.2019.01.003] |
| Science?Signaling | HIPK2 is necessary for type I interferon–mediated antiviral immunity [Doi: 10.1126/scisignal.aau4604] |
| INTERNATIONAL MEDICAL RESEARCH | Evaluation of serum interferons in patients with age-related macular degeneration [Pubmed: 31007694] |
| Veterinary Immunology and Immunopathology | Differential expression of type I interferon mRNA and protein levels induced by virulent Marek's disease virus infection in chickens [] |
| International journal of biological macromolecules | Porcine FcγRIIb mediated PRRSV ADE infection through inhibiting IFN-β by cytoplasmic inhibitory signal transduction [Pubmed: 31284005] |
| Journal of virology | Marek's Disease Virus RLORF4 Inhibits Type I Interferon Production by Antagonizing NF-κB Activation [Pubmed: 31243133] |
| The Journal of Latvian Academy of Sciences | Serodiagnosis of Human Bocavirus 1 Infection among Hospitalised Children with Lower Respiratory Tract Infection in Latvia [] |
| Cancer Medicine | The immunostimulatory effects and pro‐apoptotic activity of rhCNB against Lewis lung cancer is mediated by Toll‐like receptor 4 [Pubmed: 31218844] |
| FASEB Journal | Scavenger receptor A impairs interferon response to HBV infection by limiting TRAF 3 ubiquitination through recruiting OTUB 1 [] |
| PLOS Pathogens | Avian oncogenic herpesvirus antagonizes the cGAS-STING DNA-sensing pathway to mediate immune evasion [Pubmed: 31539404] |
| Autophagy | Induction of autophagy and suppression of type I IFN secretion by CSFV [Pubmed: 32160078] |
| MOLECULAR IMMUNOLOGY | BVDV-1 induces interferon-beta gene expression through a pathway involving IRF1, IRF7, and NF-κB activation [Pubmed: 33053462] |
| Autophagy | LDHB inhibition induces mitophagy and facilitates the progression of CSFV infection [Pubmed: 32924761] |
| Arch Virol . | Infectious bronchitis virus inhibits activation of the TLR7 pathway, but not the TLR3 pathway [Pubmed: 32532134] |
| Virulence | Determination of antiviral action of Long Non-coding RNA loc107051710 During Infectious Bursal Disease Virus Infection due to Enhancement of Interferon?… [] |
| bioRxiv | Transferrin-dependent crosstalk between the intestinal tract and commensal microbes contributes for immune tolerance [] |
| Res Vet Sci | Open reading frame 5 protein of porcine circovirus type 2 induces RNF128 (GRAIL) which inhibits mRNA transcription of interferon-β in porcine epithelial cells [34416463] |
| Eur J Immunol | Anti‐IFN‐α/‐ω neutralizing antibodies from COVID‐19 patients correlate with downregulation of IFN response and laboratory biomarkers of disease severity [Pubmed:35419822] |
| ResearchSquare | Chicken Intestinal Microbiota Modulation of Resistance to Nephropathogenic Infectious Bronchitis Virus Infection Through IFN-I [] |
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