癌胚抗原(CEA)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit for Carcinoembryonic Antigen (CEA)
CD66E; CD66; CEACAM5; Carcinoembryonic Antigen-related Cell Adhesion Molecule 5; Carcinoembryonic Antigen
- 編號(hào)USEA150Si
- 物種Rhesus monkey (Simian，恒河猴) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時(shí)長(zhǎng)3h
- 檢測(cè)范圍23.44-1,500pg/mL
- 靈敏度最小可檢測(cè)劑量小于等于7.97pg/mL.
- 樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說(shuō)明書 中文說(shuō)明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價(jià)格 ¥ 1512 ¥ 2160 ¥ 9720 ¥ 18360 ¥ 151200
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實(shí)驗(yàn)結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測(cè)癌胚抗原(CEA)，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來(lái)源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè)，因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的癌胚抗原(CEA)（加標(biāo)樣品），重復(fù)測(cè)定并計(jì)算其均值，回收率為測(cè)定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 87-97 | 93 |
| EDTA plasma(n=5) | 82-104 | 90 |
| heparin plasma(n=5) | 78-105 | 81 |
精密度
精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定，每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的癌胚抗原(CEA)，并倍比稀釋成1:2，1:4，1:8，1:16的待測(cè)樣本，線性范圍即為稀釋后樣本中癌胚抗原(CEA)含量的測(cè)定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 89-96% | 83-91% | 92-101% | 92-99% |
| EDTA plasma(n=5) | 89-96% | 83-96% | 89-97% | 95-104% |
| heparin plasma(n=5) | 78-94% | 98-105% | 78-102% | 93-105% |
穩(wěn)定性
經(jīng)測(cè)定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來(lái)進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時(shí)；
3. 吸棄，加檢測(cè)溶液A100µL，37°C孵育1小時(shí)；
4. 洗板3次；
5. 加檢測(cè)溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實(shí)驗(yàn)原理
將癌胚抗原(CEA)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的癌胚抗原(CEA)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的癌胚抗原(CEA)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過(guò)氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的癌胚抗原(CEA)呈正相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度(O.D.值)，計(jì)算樣品濃度。
贈(zèng)品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Rhesus monkey (Simian，恒河猴) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| USEA150Si | 癌胚抗原(CEA)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA150Si | 癌胚抗原(CEA)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| BioFactors | Ginger ingredients inhibit the development of diethylnitrosoamine induced premalignant phenotype in rat chemical hepatocarcinogenesis model [PubMed: 20872761] |
| Life Sciences | Comparison of angiotensin converting enzyme inhibitors and angiotensin II type 1 receptor blockade for the prevention of premalignant changes in the liver [ScienceDirect: S0024320511002700] |
| Iranian journal of basic medical sciences | Saffron Aqueous Extract Inhibits the Chemically-induced Gastric Cancer Progression in the Wistar Albino Rat [PubMed: PMC3637902] |
| Biosensors and Bioelectronics | Handy, rapid and multiplex detection of tumor markers based on encoded silica-hydrogel hybrid beads array chip [Pubmed: 23672876] |
| Asian Pac J Cancer Prev. | Association of Paraoxonase-1 (Q192R and L55M) Gene Polymorphisms and Activity with Colorectal Cancer and Effect of Surgical Intervention. [Pubmed:25684529] |
| Journal of Analytical Atomic Spectrometry | Detection of HIV-1 p24 antigen using streptavidin–biotin and gold nanoparticles based immunoassay by inductively coupled plasma mass spectrometry [Rsc:Source] |
| Breast | Activated Leukocyte Cell Adhesion Molecule (ALCAM) in Saudi Breast Cancer Patients as Prognostic and Predictive Indicator [PubMed: 26446295] |
| The Journal of Toxicological Sciences | Gene expression analysis in the lung of the rasH2 transgenic mouse at week 4 prior to induction of malignant tumor formation by urethane and N-methylolacrylamide [PubMed: 26558449] |
| PLoS?One | Collecting Protein Biomarkers in Breath Using Electret Filters: A Preliminary Method on New Technical Model and Human Study [Pubmed:26934615] |
| Journal of Applied Pharmaceutical Science | Chemopreventive effect of Indigofera linnaei extract against diethylnitrosamine induced hepatocarcinogenesis in rats [2071_pdf.pdf] |
| Analytical chemistry | Temporal Sensing Platform Based on Bipolar Electrode for the Ultrasensitive Detection of Cancer Cells [pubmed:27506255] |
| Frontiers in Physiology? | Anticarcinogenic potential of ethanol extract of Indigofera cordifolia Roth.(Fabales: Fabaceae) on diethylnitrosamine induced hepatocarcinogenesis in rats [v04n07a07a] |
| Analytica?Chimica?Acta | Ultrasensitive cathode photoelectrochemical immunoassay based on TiO2 photoanode-enhanced 3D Cu2O nanowire array photocathode and signal amplification by?… [Pubmed:29866267] |
| Journal of?Cancer | Evaluation of Detection Methods and Values of Circulating Vascular Endothelial Growth Factor in Lung Cancer [Pubmed:29675110] |
| Clinical?Breast?Cancer | 8-Hydroxy-2'-deoxyguanosine as a Discriminatory Biomarker For Early Detection of Breast Cancer [] |
| Journal of Ethnopharmacology | Preventive potential of dietary inclusion of Brachystegia eurycoma (Harms) seeds on N-methyl-N-nitrosourea-induced colon carcinogenesis in Wistar rats [Pubmed: 30953819] |
| Asian Journal of Pharmacy and Pharmacology | Chemopreventive effects of on diethylnitrosamine induced and Indigofera cassioides phenobarbital promoted rat liver carcinoma [] |
| Biomaterials | DNA-templated porous nanoplatform towards programmed “double-hit” cancer therapy via hyperthermia and immunogenicity activation [Pubmed: 31374481] |
| Biology | Piperine Regulates Nrf-2/Keap-1 Signalling and Exhibits Anticancer Effect in Experimental Colon Carcinogenesis in Wistar Rats [Pubmed: 32967203] |
| Oncology Research and Treatment | Evaluation of the Diagnostic and Predicative Values of 8-Iso-Prostaglandin F2α as a Biomarker of Breast Cancer [Pubmed: 32721979] |
| Journal of Functional Foods | Chemopreventive effect of dietary inclusion with Crassocephalum rubens (Juss ex Jacq) leaf on N-methyl-N-nitrosourea (MNU)-induced colorectal carcinogenesis in?… [] |
| Lateral Flow Immunosensor for Ferritin Based on Dual Signal-Amplified Strategy by Rhodium Nanoparticles [] | |
| Anti-Cancer Activity of Piperine Against Colon Carcinogenesis Via Modulation of NF-κB/Nrf-2/Keap1/HO-1 Signalling Pathways [] | |
| Microchemical Journal | Magnetic immunoassay for tumor clinical diagnosis based on rolling circular amplification (RCA) coupled with ICP-MS [] |
| Anticancer efficacy of β-Sitosterol Loaded Hydroxyapatite-Alginate on Colon Cancer Cell in Vivo [] | |
| sensors and actuators b-chemical | A light-induced self-powered competitive immunosensor for the detection of platelet derived growth factor-BB via an elaborately assembled bioconjugate [] |
