多巴胺(DA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Dopamine (DA)
2-(3,4-Dihydroxyphenyl)ethylamine; 3,4-Dihydroxyphenethylamine; 3-Hydroxytyramine; Intropin; Revivan; Oxytyramine; Dopamine Hydrochloride
- 編號UCEA851Ge
- 物種Pan-species (General，通用) 相同的名稱，不同的物種。
- 實驗方法競爭抑制
- 反應(yīng)時長2h
- 檢測范圍12.35-1,000pg/mL
- 靈敏度最小可檢測劑量小于等于4.71pg/mL.
- 樣本類型Serum, plasma and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 1671 ¥ 2387 ¥ 10741 ¥ 20288 ¥ 167076
- 欲了解實際交易價格和更多情況，請與當(dāng)?shù)亟?jīng)銷商聯(lián)系!
-
產(chǎn)品包裝(模擬)
-
產(chǎn)品包裝(模擬)
-
實驗結(jié)果圖
-
標(biāo)準(zhǔn)曲線圖
-
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測多巴胺(DA)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的多巴胺(DA)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 95-102 | 99 |
| EDTA plasma(n=5) | 86-93 | 89 |
| heparin plasma(n=5) | 90-99 | 94 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的多巴胺(DA)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中多巴胺(DA)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 94-103% | 94-105% | 97-104% | 85-104% |
| EDTA plasma(n=5) | 78-102% | 92-105% | 80-99% | 79-101% |
| heparin plasma(n=5) | 91-99% | 85-103% | 78-93% | 80-101% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）50µL，
加入50µL檢測液A(臨用前配制);
37°C溫育1小時。
3. 洗板3次；
4. 加檢測溶液B100µL，37°C孵育30分鐘；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分鐘；
7. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
本試劑盒應(yīng)用競爭抑制酶聯(lián)免疫分析法測定標(biāo)本中待測物質(zhì)水平。將多巴胺抗體包被微孔板，制成固相載體，往包被抗體的微孔中同時加入生物素標(biāo)記的抗原和待測抗原（標(biāo)準(zhǔn)品或樣本），待測抗原與生物素標(biāo)記抗原對特異性抗體進行競爭結(jié)合。溫育后經(jīng)洗滌去掉未結(jié)合物，然后加入HRP標(biāo)記的親和素，經(jīng)過溫育和徹底洗滌后加入底物TMB顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。待測標(biāo)本濃度越高，標(biāo)記抗原和抗體的結(jié)合就越受到抑制，顯色愈淺。顯色的深淺與酶量呈正相關(guān)，而與樣品中待測物質(zhì)含量呈負(fù)相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度（O.D.值），計算樣品濃度。
相關(guān)產(chǎn)品
| 編號 | 適用物種：Pan-species (General，通用) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UCPA851Ge41 | 多巴胺(DA)人血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UCPA851Ge11 | 多巴胺(DA)牛血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UCPA851Ge21 | 多巴胺(DA)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UPAA851Ge01 | 多巴胺(DA)多克隆抗體 | ELISA |
| ULAA851Ge71 | 多巴胺(DA)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| ULAA851Ge81 | 多巴胺(DA)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| UMAA851Ge21 | 多巴胺(DA)單克隆抗體 | ELISA |
| UMEA851Ge | 多巴胺(DA)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UCEA851Ge | 多巴胺(DA)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UIEA851Ge | 多巴胺(DA)檢測試劑盒(酶聯(lián)免疫吸附試驗法,快捷型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UCCA851Ge | 多巴胺(DA)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA851Ge | 多巴胺(DA)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA851Ge11 | 多巴胺(DA)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Renal Failure | Circulating Levels of Renalase, Norepinephrine, and Dopamine in Dialysis Patients [Pubmed: 23530612] |
| Molecular Neurobiology | Ursodeoxycholic Acid Ameliorates Apoptotic Cascade in the Rotenone Model of Parkinson’s Disease: Modulation of Mitochondrial Perturbations [Pubmed:25502462] |
| Molecular Neurobiology | Bone Marrow-Derived Endothelial Progenitor Cells Protect Against Scopolamine-Induced Alzheimer-Like Pathological Aberrations [Pubmed:25526861] |
| Cardiology. | Effect of salt intake and potassium supplementation on urinary renalase and serum dopamine levels in Chinese adults [Pubmed:25824645] |
| Eur J Pharmacol | Nalbuphine could decrease the rewarding effect induced by tramadol in mice while enhancing its antinociceptive activity [PubMed: 25843409] |
| Neuropsychiatr Dis Treat | Catha edulis chewing effects on treatment of paranoid schizophrenic patients [PubMed: 25926735] |
| Journal of Neurochemistry | Neuroprotective effects of vildagliptin in rat rotenone Parkinson's disease model: role of RAGE‐NFκB and Nrf2‐antioxidant signaling pathways [PubMed: 25752913] |
| Beni-Suef University Journal of Basic and Applied Sciences | Modulatory effect of cilostazol on tramadol-induced behavioral and neurochemical alterations in rats challenged across the forced swim despair test [science:S2314853516300166] |
| Neuroscience | Imipramine and amitriptyline ameliorate the rotenone model of Parkinson's disease in rats [pubmed:27365173] |
| Metab Brain Dis. | Neuroprotective effect of Cucumis melo Var. flexuosus leaf extract on the brains of rats with streptozotocin-induced diabetes. [pubmed:27488111] |
| Nutrition | Ginkgo biloba extract alleviates oxidative stress and some neurotransmitters changes induced by aluminum chloride in rats [S0899900716302350] |
| Nutrition | Ginkgo Biloba Extract Alleviates Oxidative Stress and Some Neurotransmitters Changes Induced by Aluminum Chloride in Rats [pubmed:28241996] |
| Effects of Junk Foods on Brain Neurotransmitters (Dopamine and Serotonin) and some Biochemical Parameters in Albino Rats [article_1486_c0fa9f8afe5f70b407e7195bcfd55c70] | |
| Behavioural?Brain?Research | High Fat Diet feeding induced insulin resistance exacerbates 6-OHDA mediated neurotoxicity and behavioral abnormalities in rats [Pubmed:29842916] |
| Toxicology?Reports | Neuro-and nephroprotective effect of grape seed proanthocyanidin extract against carboplatin and thalidomide through modulation of inflammation, tumor?… [Pubmed:29854627] |
| Molecular and Cellular Biochemistry | Repetitive transcranial magnetic stimulation inhibits Sirt1/MAO-A signaling in the prefrontal cortex in a rat model of depression and cortex-derived astrocytes [Pubmed:28948423] |
| Physiology &?Behavior? | Linagliptin potentiates the effect of l-dopa on the behavioural, biochemical and immunohistochemical changes in experimentally-induced Parkinsonism: Role of toll?… [Pubmed:29410018] |
| Behavioural?Brain?Research | Possible mechanisms of prenatal cold stress induced-anxiety-like behavior depression in offspring rats [Pubmed: 30423388] |
| Indian?Journal of Ophthalmology | Dopamine levels in human tear fluid [Pubmed: 30574889] |
| Journal of Taibah University for Science | Neurotoxicity and inflammation induced by individual and combined exposure to iron oxide nanoparticles and silver nanoparticles [] |
| Metabolic Brain Disease | Soya bean rich diet is associated with adult male rat aggressive behavior: relation to RF amide-related peptide 3-aromatase-neuroestrogen pathway in the brain [Pubmed: 31134480] |
| Functional activity of p-glycoprotein in blood-brain barrier during experimental par-kinson's syndrome [] | |
| Neurotoxicity of Mn3O4 nanoparticles: Apoptosis and dopaminergic neurons damage pathway [Pubmed: 31740235] | |
| Neurochemistry International | Polydatin protects SH-SY5Y in models of Parkinson's disease by promoting Atg5-mediated but parkin-independent autophagy [Pubmed: 31926197] |
| Neurochemistry International | Iron overload induced by IRP2 gene knockout aggravates symptoms of Parkinson's disease [Pubmed: 31904393] |
| Journal of neural transmission (Vienna, Austria : 1996) | Neurotransmitter system aberrations in patients with drug addiction [Pubmed: 32804296] |
| Acupunct Med | Electroacupuncture alleviates intestinal inflammation and barrier dysfunction by activating dopamine in a rat model of intestinal ischaemia [Pubmed: 32517478] |
| Effect of metformin and indole-3-carbinol on a rat model of Parkinson's disease induced by 6-hydroxydopamine [] | |
| South African Journal of Botany | Neuroprotective effects of pulicaria undulata essential oil in rotenone model of parkinson's disease in rats: Insights into its anti-inflammatory and anti-oxidant effects [] |
| ACTA PHARMACOLOGICA SINICA | The vascular endothelial growth factor trap aflibercept induces vascular dysfunction and hypertension via attenuation of eNOS/NO signaling in mice [33303990] |
| Neural Regeneration Research | Glucagon-like peptide-1/glucose-dependent insulinotropic polypeptide dual receptor agonist DA-CH5 is superior to exendin-4 in protecting neurons in the 6??- [33433498] |
| International Journal of?Nanomedicine | Water-Soluble Carbon Dots in Cigarette Mainstream Smoke: Their Properties and the Behavioural, Neuroendocrinological, and Neurotransmitter Changes They ?- [33762821] |
| CRYOBIOLOGY | O-GlcNAc/Akt pathway regulates glucose metabolism and reduces apoptosis in liver of piglets with acute cold stress [33651993] |
| PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | A pair of dopamine neurons mediate chronic stress signals to induce learning deficit in Drosophila melanogaster [34654742] |
| ACS Chem Neurosci | Neuroprotective Effects of Trehalose and Sodium Butyrate on Preformed Fibrillar Form of α-Synuclein-Induced Rat Model of Parkinson's Disease [34197084] |
| Exp Brain Res | Effects of early life stress during stress hyporesponsive period (SHRP) on anxiety and curiosity in adolescent rats [Pubmed:35141770] |
