免疫球蛋白G(IgG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Immunoglobulin G (IgG)
- 編號UCEA544Gu
- 物種Cavia (Guinea pig ，豚鼠) 相同的名稱，不同的物種。
- 實驗方法競爭抑制
- 反應(yīng)時長2h
- 檢測范圍1.23-100ug/mL
- 靈敏度最小可檢測劑量小于等于0.57ug/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 920 ¥ 1314 ¥ 5913 ¥ 11169 ¥ 91980
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結(jié)果圖
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標準曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測免疫球蛋白G(IgG)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的免疫球蛋白G(IgG)（加標樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 86-101 | 95 |
| EDTA plasma(n=5) | 90-102 | 97 |
| heparin plasma(n=5) | 84-91 | 87 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的免疫球蛋白G(IgG)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中免疫球蛋白G(IgG)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 79-96% | 80-98% | 86-105% | 89-101% |
| EDTA plasma(n=5) | 86-99% | 95-102% | 86-95% | 93-101% |
| heparin plasma(n=5) | 84-101% | 92-101% | 78-96% | 87-104% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）50µL，
加入50µL檢測液A(臨用前配制);
37°C溫育1小時。
3. 洗板5次；
4. 加TMB底物90µL，37°C孵育10-20分鐘；
5. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
本試劑盒應(yīng)用競爭抑制酶聯(lián)免疫分析法測定標本中待測物質(zhì)水平。將免疫球蛋白G(IgG)抗體包被微孔板，制成固相載體，往包被抗體的微孔中同時加入過氧化物酶標記的抗原和待測抗原(標準品或樣本)，待測抗原與酶標記抗原對特異性抗體進行競爭結(jié)合。經(jīng)過溫育和徹底洗滌后加入底物TMB顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成最終的黃色。待測標本濃度越高，標記抗原和抗體的結(jié)合就越受到抑制，顯色愈淺。顯色的深淺與酶量呈正相關(guān)，而與樣品中待測物質(zhì)含量呈負相關(guān)。用酶標儀在450nm波長下測定吸光度（O.D.值），計算樣品濃度。
相關(guān)產(chǎn)品
| 編號 | 適用物種：Cavia (Guinea pig ，豚鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UNPA544Gu01 | 免疫球蛋白G(IgG)天然蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| USAA544Gu00 | 兔抗豚鼠IgG多克隆抗體(無標記) | WB |
| UPAA544Gu01 | 免疫球蛋白G(IgG)多克隆抗體 | WB |
| USAA544Gu06 | 兔抗豚鼠IgG多克隆抗體(吲哚菁綠標記) | WB; IHC; ICC; IF. |
| USAA544Gu05 | 兔抗豚鼠IgG多克隆抗體(別藻藍蛋白標記) | WB; IHC; ICC; IF. |
| USAA544Gu04 | 兔抗豚鼠IgG多克隆抗體(藻紅素標記) | WB; IHC; ICC; IF. |
| USAA544Gu09 | 兔抗豚鼠IgG多克隆抗體(辣根過氧化物酶標記) | WB |
| USAA544Gu07 | 兔抗豚鼠IgG多克隆抗體(生物素標記) | WB; IHC; ICC. |
| USAA544Gu08 | 兔抗豚鼠IgG多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| UMAA544Gu21 | 免疫球蛋白G(IgG)單克隆抗體 | WB |
| UCEA544Gu | 免疫球蛋白G(IgG)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UCCA544Gu | 免疫球蛋白G(IgG)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA544Gu | 免疫球蛋白G(IgG)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA544Gu11 | 免疫球蛋白G(IgG)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
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