肌動蛋白β(ACTB)單克隆抗體
Monoclonal Antibody to Beta Actin (ACTB)
Actin Beta; ACT-B; PS1TP5BP1; Actin, cytoplasmic 1; Actin, cytoplasmic 1, N-terminally processed
- 編號UMAB340Mi22
- 物種Homo sapiens (Human，人), Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠), Canis familiaris; Canine (Dog，犬), Sus scrofa; Porcine (Pig，豬), Bos taurus; Bovine (Cattle，牛) 相同的名稱，不同的物種。
- 來源單抗制備
- 宿主小鼠
- 效價(克隆號)C5
- Ig亞型 IgG2b Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標記物無標記物
- 免疫原 RPB340Mi01-肌動蛋白β(ACTB)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度1mg/mL
- 且適物種Homo sapiens (Human，人), Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠), Oryctolagus cuniculus (Rabbit，兔), Canis familiaris; Canine (Dog，犬), Sus scrofa; Porcine (Pig，豬), Bos taurus; Bovine (Cattle，牛)
- 應(yīng)用WB; IHC
如果抗體需用于流式細胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 376 ¥ 878 ¥ 1254 ¥ 3135 ¥ 12540
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Sample: Human Tonsil Tissue; Primary Ab: 10μg/ml Mouse Anti-Multi-species ACTb Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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DAB staining on IHC-P;
Sample: Human Kidney Tissue;
Primary Ab: 20μg/ml Mouse Anti-Multi-species ACTb Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Tonsil Tissue
Primary Ab: 10μg/ml Mouse Anti-Multi-species ACTB Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Kidney Tissue
Primary Ab: 10μg/ml Mouse Anti-Multi-species ACTB Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Porcine Kidney Tissue
Primary Ab: 20μg/ml Mouse Anti-Multi-species ACTB Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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Western Blot; Sample: Lane1: Human Lung lysate; Lane2: Hela cell lysate Primary Ab: 3μg/ml Mouse Anti-Multi-species ACTb Antibody Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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Western Blot; Sample: Lane1: Human Lung lysate; Lane2: 293T cell lysate; Lane3: Rat Liver lysate; Lane4: Rat Kidney lysate; Lane5: Porcine Liver lysate; Lane6: Porcine Kidney lysate; Lane7: Mouse Liver lysate; Lane8: Mouse Kidney lysate; Lane9: Rabbit Liver lysate; Lane10: Bovine Kidney lysate; Lane11: Canine Kidney lysate
Primary Ab: 1μg/ml Mouse Anti-Multi-species ACTb Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
該抗體是針對ACTB的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別ACTB。
用法
Western blotting: 0.01-3μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人), Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠), Canis familiaris; Canine (Dog，犬), Sus scrofa; Porcine (Pig，豬), Bos taurus; Bovine (Cattle，牛) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPB340Mi01 | 肌動蛋白β(ACTB)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UCAB340Mi01 | 抗肌動蛋白β(ACTB)多克隆抗體 | WB |
| UPAB340Mi01 | 肌動蛋白β(ACTB)多克隆抗體 | WB; IHC |
| UMAB340Mi22 | 肌動蛋白β(ACTB)單克隆抗體 | WB; IHC |
| UCAB340Mi22 | 抗肌動蛋白β(ACTB)單克隆抗體 | WB |
| UMAB340Mi23 | 肌動蛋白β(ACTB)單克隆抗體 | WB; IHC; ICC/IF |
| UMAB340Mi21 | 肌動蛋白β(ACTB)單克隆抗體 | WB; IHC |
| UMAB340Mi24 | 肌動蛋白β(ACTB)單克隆抗體 | WB |
| USEB340Mi | 肌動蛋白β(ACTB)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCB340Mi | 肌動蛋白β(ACTB)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMB340Mi | 肌動蛋白β(ACTB)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSB340Mi01 | 肌動蛋白β(ACTB)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
| UPGB340Mi01 | 肌動蛋白β(ACTB)引物對 | PCR |
參考文獻
| 雜志 | 參考文獻 |
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| bioscience biotechnology research asia | Malted Barley Improved the Structure and Function of Gastrocnemius Muscle of Hypercholesterolemic Mother Rats and their Offspring [] |
| Genes | Tryptophan Depletion Modulates Tryptophanyl-tRNA Synthetase-Mediated High-Affinity Tryptophan Uptake into Human Cells [33261077] |
| Nat Commun | POLRMT mutations impair mitochondrial transcription causing neurological disease [33602924] |
| Life Sci | Indole-linked 1, 2, 3-triazole derivatives efficiently modulate COX-2 protein levels in human THP-1 monocytes by suppressing AGE-ROS-NF-kβ nexus [Pubmed:34990649] |
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